By Krishnarao Appasani, Sir E.M. Southern
This ebook presents an built-in number of well timed articles at the use of bioarray suggestions within the fields of biotechnology and molecular drugs. it's the first ebook to comprehensively combine molecular diagnostics and molecular pathology. This ebook serves as an crucial reference for graduate scholars, post-docs, and professors in addition to an explanatory research for executives and scientists in biotechnology and pharmaceutical businesses.
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Additional resources for Bioarrays: From Basics to Diagnostics
11. Jongeneel, C. , Stevenson, B. , et al. (2003) Comprehensive sampling of gene expression in human cell lines with massively parallel signature sequencing. Proc. Natl. Acad. Sci. USA 100, 4702–4705. 12. , Young, M. , et al. (2003) Gene expression profile of human bone marrow stromal cells: high-throughput expressed sequence tag sequencing analysis. Genomics 79, 7–17. 13. , Davis, R. , and Brown, P. O. (1995) Quantitative monitoring of gene expression patterns with a complementary DNA microarray.
Multiple levels of technical problems, such as sample preparation, array spotting, signal acquisition, dye intensity bias, normalization, or sample-contamination, can generate inconsistent results or misleading conclusions. Technical recommendations that offer alternatives and solutions for the most common problems have been discussed extensively in previous work. Less often discussed is the experimental design. A poor design can make array data analysis difficult, even if there are no technical problems.
28 Carraro et al. 46. , and Bralet, M. P. (2002) Tissue microarrays, or the advent of chips in pathology. Ann. Pathol. 22, 69–72. 47. , and Fuller, G. N. (2002) Tissue microarrays: applications in neuropathology research, diagnosis, and education. Brain Pathol. 12, 95–107. Experimental Design for Gene Expression Analysis 29 3 Experimental Design for Gene Expression Analysis Answers Are Easy, Is Asking the Right Question Difficult? Marcia V. Fournier, Paulo Costa Carvalho, David D. Magee, Maria da Gloria Costa Carvalho, and Krishnarao Appasani Summary More and more, array platforms are being used to assess gene expression in a wide range of biological and clinical models.