Download Cellular Regulators Part A: Calcium- and Calmodulin-Binding by Anthony R. Means, P. Michael Conn (Eds.) PDF

By Anthony R. Means, P. Michael Conn (Eds.)

The seriously acclaimed laboratory commonplace, Methods in Enzymology, is likely one of the such a lot hugely revered courses within the box of biochemistry. for the reason that 1955, every one quantity has been eagerly awaited, usually consulted, and praised by means of researchers and reviewers alike. The sequence comprises a lot fabric nonetheless correct this day - really a necessary ebook for researchers in all fields of lifestyles sciences

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1° Recent studies carried out in our laboratory (unpublished observations) on over a dozen animal tissues from many phyla suggest that calcimedins have a universal distribution but vary in concentration and ratio in different tissues. Procedure All chemicals were reagent grade. DE-52 was purchased from Whatman Laboratory Sales, Inc. (Hilsboro, OR); phenyl-Sepharose, aprotinin, and molecular weight standards were obtained from Sigma Chemical Company (St. Louis, MO). The source of Ultrogel AcA 44 was LKB (Gaithersburg, MD).

S F. Silva, K. Sherril, S. Spurgeon, T. Siidhof,and D. K. Stone, Differentiation (1986) (in press). 9T. C. Sfidhof,C. W. Zimmerman,and J. H. Walker, Fur. J. Cell Biol. 30, 214 (1983). toj. H. Walker, J. Obrocki, and T. C. S~idhof,J. Neurochem. 41, 139 (1983). n M. Geisow, J. Childs, B. Dash, A. Harris, G. Pancryotow,T. C. Siidhof, and J. H. Walker, EMBO J. 3, 2969 (1984). n T. C. Siidhof,unpublishedobservation(1984). 4. From this column calelectrin elutes as a single peak at a position corresponding to its monomeric molecular weight and is homogeneous by SDS polyacrylamide gel electrophoresis.

Spurgeon, T. Siidhof,and D. K. Stone, Differentiation (1986) (in press). 9T. C. Sfidhof,C. W. Zimmerman,and J. H. Walker, Fur. J. Cell Biol. 30, 214 (1983). toj. H. Walker, J. Obrocki, and T. C. S~idhof,J. Neurochem. 41, 139 (1983). n M. Geisow, J. Childs, B. Dash, A. Harris, G. Pancryotow,T. C. Siidhof, and J. H. Walker, EMBO J. 3, 2969 (1984). n T. C. Siidhof,unpublishedobservation(1984). 4. From this column calelectrin elutes as a single peak at a position corresponding to its monomeric molecular weight and is homogeneous by SDS polyacrylamide gel electrophoresis.

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