Download Complex Carbohydrates Part B by Victor Ginsburg (Eds.) PDF

By Victor Ginsburg (Eds.)

The significantly acclaimed laboratory common, Methods in Enzymology, is likely one of the such a lot hugely revered guides within the box of biochemistry. given that 1955, each one quantity has been eagerly awaited, often consulted, and praised by means of researchers and reviewers alike. The sequence includes a lot fabric nonetheless appropriate this day - really an important e-book for researchers in all fields of lifestyles sciences

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Complex Carbohydrates Part B

The seriously acclaimed laboratory commonplace, tools in Enzymology, is among the such a lot hugely revered guides within the box of biochemistry. in view that 1955, each one quantity has been eagerly awaited, usually consulted, and praised by means of researchers and reviewers alike. The sequence comprises a lot fabric nonetheless suitable at the present time - really a necessary booklet for researchers in all fields of lifestyles sciences

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Gottschalk, Biochem. Biophys. Res. Commun. 16, 333 (1964). K. Tanaka and W. Pigman, J. Biol. Chem. 240, PC 1487 (1965). 36 :NAL:T:CXL ME~HODS [1] charities). , galactitol, galactosaminitol, xylitol, or mannitol). The amino sugar alcohol can be identified on the amino acid analyzer with the citrate-borate system. 81, and the Rxy! 87, as well as by gas-liquid chromatography as their acetates. Reduction with sodium borohydride and palladium chloride of the unsaturated amino acid formed during fl-elimination makes possible the identification of the amino acid involved in the glycopeptide bond not only by its decrease after the alkaline treatment, but also by the increase in alanine from serine through the reduction of the a-aminoacrylic acid and the appearance of a-aminobutyric acid (which elutes between alanine and glucosamine on the Technicon amino acid analyzer) from threonine through reduction of a-aminocrotonic acid (see also [3]).

J. Gorin and A. J. Finlayson, Carbohyd. Res. 18, 269 (1971). ~'P. A. J. Gorin, Carbohyd. Res. 18, 281 (1971). "A. Tarentino, T. H. , and F. Maley, J. Biol. Chem. 245, 4150 (1970). A. B. Foster, D. Horton, N. Salim, M. Stacey, and J. M. Webber, J. Chem. Soc. 2587 (1960). [1] THE CARBOHYDR&TES OF GLYCOPROTEINS 29 TABLE V AMINO POLYOLS OBTAINED FROM OLIGOSACCHARIDES TERMINATING IN N-AcETYLHEXOSAMINITOL AFTER PERIODATE OXIDATION AND BOROHYDRIDE I:~EDUCTIONa Terminal hexosaminitol Linkage to hexosaminitol N-Acetyl-D-glucosaminitol N-Acetyl-D-galactosaminitol 1 -~ 3 1 ---*4 1 --* 6 1 --~ 3 plus 1 -~ 6 1 -* 3 plus 1 --~ 4 1 --~ 4 plus 1 -~ 6 L-Threosaminitol D-Xylosaminitol Serinol n-Threosaminitol D-Xylosaminitol D-Glucosaminitol L-Threosaminitol L-Arabinosaminitol Serinol L-Threosaminitol L-Arabinosaminitol D-Galactosaminitol a The reduced oxidation products are hydrolyzed in acid to obtain the amino polyols (see text).

Chem. 246, 3259 (1971). k R. Kornfeld and S. Kornfeld, J. Biol. Chem. 245, 2536 (1970). z R. G. Spiro and V. D. Bhoyroo, unpublished observations (1971). "* K. M. L. Agrawal and O. P. Bahl, J. Biol. Chem. 243, 103 (1968). n O. P. Bahl, J. Biol. Chem. 244, 575 (1969). ° P. V. Wagh, ][. Bornstein, and R. J. Winzler, J. Biol. Chem. 244, 658 (1969). O. P. Bahl and K. M. L. Agrawal, J. Biol. Chem. 244, 2970 (1969). S. Chipowsky and E. J. McGuire, Fed. , Fed. Amer. Soc. Exp. Biol. 28, 606 (1969). r L.

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