By A. Garcia-Bellido (auth.), O. Siddiqi, P. Babu, Linda M. Hall, Jeffrey C. Hall (eds.)
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Additional resources for Development and Neurobiology of Drosophila
M(2)173 is a mutation leading to moderate Minute phenotype that is cytologically normal (Figure la) . Two other Minutes, M(2)017 and M(2)U were found to non-complement each other as well as M(2)173. Of these, M(2)017 was found to carry a small deletion in the 56F5-l5 region (Figures lb and c). Since it was viable when heterozygous with the aneuploid deficiency extending from 56E to 56F, it was concluded that M(2)017 is not deficient for 5S ribosemal RNA genes, which are located in the 56E to 56F segments (Procunier and Tartof, 1975).
1979, Genes for the 70,000 dalton heat shock protein in two cloned D. melanogaster DNA segments, Cell, 17:9. , 1970, Patterns of puffing activity in salivary glands of Drosophila V. Responses to environmental treatment, Chromesoma, 31:356. , 1979, The induction of gene activity in Drosophila by heat shock, Cell, 17:241. , 1977, Polytene chromosome puffing and in situ hybridization measure different aspects of RNA metabolism, Cell, 12:227. , 1969, In vitro culture of Drosophila melanogaster embryonie cells, In Vitro, 6:162.
In the F 2 , the putative lethal alleles of M(2)173 or M(2)017 were those cultures where straiqhtwinged non-Cy (b pr cn*/M) flies were absent. The lethal was collected over Cy in the surviving b pr cn*/Cy progeny and a stock was established from these flies. The F1 b pr cn*;cy was also screened for the dominant "Minute" phenotype and all of them were crossed with M(2)173 for allelism tests. All the putative alleles of M(2)173 were also crossed with both M(2)173 and M(2)017 and could be classified into three groups: (l) those that were lethal only with M(2)173; (2) those lethal only when heterozygous with M(2)017; and (3) those that were lethal when heterozygous with either M(2)173 or M(2)017.