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By C. H. W. Hirs (Eds.)

The severely acclaimed laboratory commonplace, Methods in Enzymology, is without doubt one of the such a lot hugely revered guides within the box of biochemistry. given that 1955, each one quantity has been eagerly awaited, usually consulted, and praised by means of researchers and reviewers alike. The sequence includes a lot fabric nonetheless suitable this present day - actually a vital ebook for researchers in all fields of existence sciences

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Solvents penetrating a dry layer of adsorbent are in part adsorbed; the heat of adsorption E. V. "Wiley (Interscience), New York, 1963. 5G. " de Gruyter, Berlin, 1965. aF. Geiss and H. Schlitt, Naturwissenscha]ten 50, 350 (1963). F. Geiss, H. Schlitt, F. J. Ritter, and W. M. I. Chromatog. 12, 469 (1963). 8M. Brenner, A. Niederwieser, G. Pataki, and A. R. Fahmy, Experientia 18, 101 (1962). 42 AMINO ACID ANALYSIS AND RELATED PROCEDURES [5] tO some extent converts solvent to vapor. Such evaporation induces composition changes in the gas phase and temperature fluctuations on and near the layer.

Application of Samples to Paper Of the many types of paper tested for this purpose, Whatman No. 3MM chromatography grade paper has been found to be the most satisfactory for general applications. It is available in rolls 18" wide X 300' in length. A suitable length of paper is cut from the roll and an origin line drawn across the paper at a position that will be 6" above the end of the rack after proper mounting of the paper. The samples are taken 36 AMINO ACID ANALYSIS AND RELATED PROCEDURES [4] up in a volume of water such that 10 ~I will contain 10-60 X 10-9 moles of each of the amino acids to be analyzed.

An occasional ambiguity in the interpretation of a result is more than compensated for by the versatility of the method and by the minimal requirements with regard to sample size. 1M. Brenner, A. Niederwieser, and G. Pataki, in "Thin-Layer Chromatography, A Laboratory Handbook" (E. ), p. 391. "Springer, Berlin, 1962). line. S-Carboxymethylcysteineruns slower than tryptophan, and cysteic acid moves to a point several inches below the origin. In the case of analysis of urine a large number of unidentified compounds move slower than tryptophan.

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