By Yuehuei H. An, Kylie L. Martin
Histologists and histochemists needs to spend huge attempt this day to discover the equipment necessary to practice bone and cartilage-related learn. In guide of Histology equipment for Bone and Cartilage, Y.H. An and K.L. Martin have assembled an intensive number of the foremost equipment for learning the histology and histomorphometry of bone and cartilage. defined through well-experienced researchers, physicians, and technicians, the equipment contain options for bone labeling, bone sectioning, histological research, histomorphometric research, radiological exam, and make contact with radiography. one of the functions awarded are in situ hybridization of bone and cartilage, radiological exam of calcified tissue with emphasis on bone, and working with histology artifacts in hard-tissue examine. There also are histological ideas for the research of bone-implant interfaces, cartilage-defect fix, and the soft-tissue biomaterial interface. simpler than theoretical, the textual content is easy and simple, with many tables, line drawings, and micro- and macro images to demonstrate the content material. complete bibliographies on the finish of every bankruptcy consultant the reader to extra certain information.
finished and updated, the guide of Histology equipment for Bone and Cartilage bargains researchers a realistic consultant to all of the significant histological and histomorphometrical equipment of bone and cartilage analysis-a gold mine of unique concepts for the guidance of tissue from assortment via fixation, embedding, sectioning, and staining.
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Additional info for Handbook of Histology Methods for Bone and Cartilage
Yan WQ, Nakamura T, Kobayashi M, et al: Bonding of chemically treated titanium implants to bone. J Biomed Mater Res 37:267–275, 1997. 118. Young FA, Spector M, Kresch CH: Porous titanium endosseous dental implants in Rhesus monkeys: microradiography and histological evaluation. J Biomed Mater Res 13:843–856, 1979. Experimental Bone and Cartilage Histology 31 119. Young RD, Lawrence PA, Duance VC, et al: Immunolocalization of type III collagen in human articular cartilage prepared by high-pressure cryoﬁxation, freeze-substitution, and low-temperature embedding.
Original magniﬁcation ×400. (See color plate 1 appearing in the insert following p. ) E. Staining Procedures 1. Basic and Special Staining Methods Many staining methods are available. Hematoxylin & eosin (H&E) staining remains the basic and most common procedure for most tissues. It can be used for both decalciﬁed and undecalciﬁed specimens. Both Goldner’s trichrome stain and the von Kossa stain allow differentiation of osteoid from mineralized bone matrix, although von Kossa provides little other information.
Anat Rec 247:462–471, 1997. Experimental Bone and Cartilage Histology 29 77. Paquay YC, De Ruijter AE, van der Waerden JP, et al: A one stage versus two stage surgical technique. Tissue reaction to a percutaneous device provided with titanium ﬁber mesh applicable for peritoneal dialysis. Asaio J 42:961–967, 1996. 78. Parﬁtt AM, Drezner MK, Glorieux FH, et al: Bone histomorphometry: standardization of nomenclature, symbols, and units. Report of the ASBMR Histomorphometry Nomenclature Committee. J Bone Miner Res 2:595–610, 1987.