By Nathan P. Kaplan, Nathan P. Colowick, Joel G. Hardman, Bert W. O'Malley
The seriously acclaimed laboratory commonplace, equipment in Enzymology, is without doubt one of the so much hugely revered courses within the box of biochemistry. on the grounds that 1955, every one quantity has been eagerly awaited, often consulted, and praised by means of researchers and reviewers alike. The sequence comprises a lot fabric nonetheless correct this day - actually a vital e-book for researchers in all fields of lifestyles sciences.
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The severely acclaimed laboratory typical, tools in Enzymology, is without doubt one of the such a lot hugely revered courses within the box of biochemistry. in view that 1955, every one quantity has been eagerly awaited, often consulted, and praised via researchers and reviewers alike. The sequence includes a lot fabric nonetheless suitable this present day - really an important e-book for researchers in all fields of existence sciences
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Extra resources for Hormone Action, Part C: Cyclic Nucleotides
With some lots of alumina the cyclic GMP appears in the fourth and fifth milliliter. The 2 ml desired are collected directly into a scintillation vial. To this is added 10 ml of scintillation mixture. We have successfully used both the mixture of Bray s and that of Anderson and McClure2 The radioactivity is measured in a liquid scintillation spectrometer. Interfering Compounds Ammonium sulfate, sodium phosphate or pyrophosphate, and heparin all increased the ~2p blank. However, sodium chloride, magnesium chloride, mercaptoethanol, and dithiothreitol had no effect.
C~ ~ ~ ~ ~ c~ ~ c~ • r~ -H o . ~ ~ ~ ,~ "~ . ~ ~ ~ ~ . [6 ! ALUMINA CHRO:KlkTOGRAPHY OF CYCLIC NUCLEOTIDES 41 is known to occur, especially at elevated temperature2 The combination of ZnSO4-Ba(OH)o. also gives a viscous solution in the presence of theophylline at concentrations of i0 mM or higher. 3. The use of Z n S Q - N a C Q combination is more desirable in that it does not catalyze the formation of cAMP, nor does it give a viscous supernatant with theophylline. It also gives a complete recovery of cAMP and has been used successfully in the isolation of cyclic GMP2 4.
3] S e p a r a t i o n , P u r i f i c a t i o n a n d A n a l y s i s o f C y c l i c N u c l e o t i d e s by High Pressure Ion Exchange Chromatography By GARY BROOKER High-pressure anion exchange chromatography has been used to measure cyclic AMP (cAMP) produced by adenylate cyclase1 and to measure cAMP in tissues 2 and in patient urine2 In addition, it has been shown useful in the measurement of cyclic GMP (eGMP) and cAMP analogs. 4 The anion exchange resin used in this method is that originally described by Horvath et al?