By Mingfeng Bai
This unique quantity encompasses a wealthy number of purposes utilizing a variety of instrumentations, probes, illness types, and goals with a purpose to account for the multidisciplinary nature of using in vivo fluorescence think. The booklet additionally contains chapters at the rising fields of telephone monitoring, image-guided therapy, and fluorescence imaging within the moment NIR window, in addition to protocols for evaluate tools prior to and after in vivo imaging. Written for the hugely profitable Methods in Molecular Biology sequence, chapters comprise short introductions to their respective subject matters, lists of the required fabrics and reagents, step by step effortlessly reproducible laboratory protocols, and pointers on troubleshooting and fending off identified pitfalls.
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Extra info for In Vivo Fluorescence Imaging: Methods and Protocols
Such contrast agent, however, can be readily synthesized through a one-step NaBH4 reduction from commercially available dyes [13, 14]. 1 Inflammation Imaging Contrast Agent Synthesis General Strategy A variety of NIR dyes, including the commercially available Cy7, are suitable to be used as the precursor to synthesize ROS-sensitive contrast agents . We used a Cy7 analog developed by Dr. Fluorescence Imaging of Inflammation in Live Animals 47 Scheme 1 Preparation of ROS sensitive probe LS601R-PEG40k from LS601.
6 Synthesis of melanoma-targeted fluorescence probe : (a) DMF. Reproduced with permission from Ref. 6. Copyright (2012) American Chemical Society Fig. 7 Synthesis of folate-positive KB cells targeting imaging and drug delivery system : (a) Cu(ClO4)2, sodium ascorbate, DIPEA, DMF. Reproduced with permission from Ref. 7. Copyright (2013) American Chemical Society 2. ) to the stirring mixture and heat the reaction to 70 °C and monitor the reaction (see Note 14). Remove the solvent by rotary evaporation and purify the product using the appropriate method (see Note 15).
18. The images will be saved automatically. References 1. Ferrero-Miliani L, Nielsen OH, Andersen PS, Girardin SE (2007) Chronic inflammation: importance of NOD2 and NALP3 in interleukin-1beta generation. Clin Exp Immunol 147:227–235 2. Huang J, Upadhyay UM, Tamargo RJ (2006) Inflammation in stroke and focal cerebral ischemia. Surg Neurol 66:232–245 3. Coussens LM, Werb Z (2002) Inflammation and cancer. Nature 420:860–867 4. Libby P, Ridker PM, Maseri A (2002) Inflammation and atherosclerosis. Nature 420: 868–874 5.