By A. Gorio, J.R. Perez-Polo, J. de Vellis, B. Haber
Data of all appropriate elements of neuronal mobile development and differentiation are offered during this quantity. law of expression, garage and unencumber of nerve development components, receptor regulate and the mobile responses to progress elements are comprehensively mentioned. handled also are impacts of assorted neurotransmitters on neuronal morphogenesis and new result of interactions of cells and mediators of the immune, endocrine and apprehensive approach. exact emphasis is given to these elements regulating regeneration and nerve spreading after accidents of the nerve tissue.
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Extra resources for Neural Development and Regeneration: Cellular and Molecular Aspects
Tracellular matrix, the proteoglycan chondroitin sulfate (CS). In this study, an antibody specific for the glycosaminoglycan digested with chondroitinase ABC was utilized (Bertolotto et al. 1986). In immunostained cryostat sections, CS exhibited a widespread distribution in the developing cerebellum. At the light microscopic level it was virtually impossible to determine which cell types are responsible for its synthesis, even in double immunofluorescence experiments. It was, however, interesting to note that the distribution of CS varied during development.
LITERA TURE REFERENCES Bronner-Fraser M (l986a) Analysis of the early stages of trunk neural crest migration in avian embryos using monoclonal antibody HNK-l. Develop BioI 115:44-55 30 Bronner-Fraser M (1986b) An antibody to a receptor for fibronectin and laminin perturbs cranial neural crest development in vivo. Develop Bioi 117:528-536 Ciment G, Weston JA (1985) Segregation of developmental abilities in neural crestderived cells: Identification of partially restricted intermediate cell types in the branchial arches of avian embryos.
In order to try to clarify this problem, we monitored the antigenic develop- ment of LB1 + progenitor cells in serum-containing cerebellar cultures. EFFECT OF CELL DENSITY ON THE DIFFERENTIATION PROFILE OF BIPOTENTIAL GLIAL PRECURSORS In low density, serum-containing cultures (1 x lOS cells/cm2), within S days in vitro about 70% of the A2BS +, LB1 + precursors became, as previously noted (Levi et al. 1986), GFAP+ (Fig. 1 A-B). However, even if not all, an elevated proportion of the LB1+ precursors appeared to express GFAP after having expressed the 04 antigen (Fig.