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An ee of L-Ala as low as 5% was suﬃcient to induce a CD of the same intensity as the enantiopure sample. ) and the measured CD intensity. 2 Supramolecular Aggregates Matile and co-workers79 have been particularly involved in the development of conceptually new approaches towards sensing and signal ampliﬁcation. 47 Here, the working principle is the ability of DNA to act as a carrier for hydrophilic counterions across lipid bilayers, on the prerequisite that DNA is initially coated with amphiphilic counterions in order to ‘dissolve’ DNA in the apolar bilayer.
2 Surfaces Highly sensitive analytical techniques such as SERS, SPR, and electrochemical detection all rely on interactions occurring between the analyte and a metal surface. 15 However, as will be illustrated in this section, also these sensitive surface-based detection methodologies beneﬁt from the use of signal ampliﬁcation strategies to improve their sensitivity. 44 The classical sandwich assay relies on the capture of the target oligonucleotide by a capture oligonucleotide probe immobilized on a surface.