By M.H.V. Van Regenmortel and S. Muller (Eds.)
This latest variation to the Laboratory options sequence supplies present cutting-edge use of man-made peptides in molecular biology and sensible protocols on easy methods to conjugate peptides, immunize animals with peptides and visual display unit immune responses to peptides in vitro. It supplies history info on antigenic specificity, prediction of antigenic websites in proteins and functions of peptides in immunology and virology, as probes in analysis and as vaccines. The e-book additionally describes antigenicity of proteins and techniques to localize antigenic websites in addition to equipment for predicting epitoxes, and provides distinct protocols for peptide-carrier conjugation, immunization with peptides, and peptide immunoassays. the amount additionally describes standard use of antipeptide antibodies in molecular and mobile biology in addition to using peptides within the analysis of viral infections and autoimmune illnesses, and using peptides as capability man made vaccines. a very good version to a superb sequence, on hand in hardbound and paperback.
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Extra info for Synthetic Peptides as Antigents
1991). 4. Binding studies With adpeptide antibodies In this approach, peptides are used for immunizing animals and the resulting antipeptide antibodies are tested for their capacity to crossreact with the intact protein. A positive cross-reaction is taken as evidence that the peptide approximates to an epitope of the protein. , 1983) that immunization with peptides leads to a high frequency of induction of antibodies capable of recognizing the native protein, this is no longer believed to be true.
15 D44- 1 Pheasant lysozyme Guinea-fowl lysozyme Bobwhite quail lysozyine Turkey lysozyine lnHuenza virus neurarninidase N 9 Neuraminidase N 9 InHuenza Virus X-3 I haemagglutinin E. 3 NC 41 F\ DI 3 Vanous peptide5 of 8-20 re\idues (myohaemerythrin. rnHuenza, haemagglutinin. cyclo$porm, HIV. I hinovirus. 3 FabT 91 AJ5 Chitarra et al. (1993) Lescar et al. (1995) Chacko et al. (1996) Braden et al. (1996) Tulip et al. (1992a). Tulip et al. (1992b) Malby et at. (1994) Bizebard et al. (1995) Prasad et al.
I hinovirus. 3 FabT 91 AJ5 Chitarra et al. (1993) Lescar et al. (1995) Chacko et al. (1996) Braden et al. (1996) Tulip et al. (1992a). Tulip et al. (1992b) Malby et at. (1994) Bizebard et al. (1995) Prasad et al. ( 1993) Bentley et al. (1990) Ban et at. (1994, 1995) Evans et al. ( 1 9 9 4 ~ Rose et al. (1993) Fields et al. (1995) Altschuh et al. (1992), Churchill et al. (1994), Ghiara et al. (1994). Rini et al. (1992), Rini et al. (1993), Shoham ( 1993), Stanfield et al. (1990), Tormo et al. (1994).