By I. S. Kulaev, V. M. Vagabov, T. V. Kulakovskaya(auth.)
Now in a moment variation, Biochemistry of Inorganic Polyphosphates fills the necessity for an exhaustive source on inorganic polyphosphate metabolism. The authors describe the constitution and homes of those compounds and offers a comparative research of the most recent and standard tools in their extraction from cells. Distribution of polyphosphates in organisms, their localization in cells and tissues can also be defined.
- Comprehensive presentation of inorganic polyphosphate metabolism
- Follows polyphosphates in cells of organisms from assorted phases of evolution
- Presents tools for the research and learn of polyP-dependent enzymes
- Comprehensive details on genetics, metabolism and biotechnology of polyphosphates
- Textbook and reference paintings on all facets of polyphosphates
Chapter 1 The Chemical constructions and houses of Condensed Inorganic Phosphates (pages 3–13):
Chapter 2 tools of Polyphosphate Assay in organic fabrics (pages 15–35):
Chapter three The prevalence of Polyphosphates in residing Organisms (pages 37–44):
Chapter four The varieties within which Polyphosphates are found in Cells (pages 45–51):
Chapter five Localization of Polyphosphates in Cells of Prokaryotes and Eukaryotes (pages 53–63):
Chapter 6 Enzymes of Polyphosphate Biosynthesis and Degradation (pages 65–89):
Chapter 7 The services of Polyphosphates and Polyphosphate? based Enzymes (pages 91–123):
Chapter eight The Peculiarities of Polyphosphate Metabolism in numerous Organisms (pages 125–181):
Chapter nine utilized facets of Polyphosphate Biochemistry (pages 183–191):
Chapter 10 Inorganic Polyphosphates in Chemical and organic Evolution (pages 193–209):
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Extra resources for The Biochemistry of Inorganic Polyphosphates, Second Edition
3). With regard to the quantitative determination of PolyP and PolyP chain length by using NMR spectroscopy, it must be taken into account that the total intensity of the middle phosphate groups is proportional to the concentration of each individual PolyPs with a certain chain length, but no proportional correlation is observed during the transitions between PolyPs with a small number of phosphate groups and PolyPs with a large number of these groups. The contribution of PP4 groups to the total intensity of the peak decreases with an increase in the PolyP chain length.
Thus, the cytochemical distinguishing of cell inclusions is still an interesting, but not simple, experimental task. 2HCl (DAPI), which is commonly used for DNA detection. , 1990). DAPI–DNA fluorescence is blue–white, while DAPI–PolyP and DAPI–lipid fluorescence is yellow. , 1990). The excitation wavelength for DAPI is 330–385 nm. The emission maximum of DAPI is 456 nm; different polyaniones, such as DNA or poly(glutamic acid), induced a strong increase in the fluorescence intensity depending on the concentration.
1971). 5 and 7), it is possible to distinguish between the condensed phosphates, which differ in their polymerization degrees or are bound with different compounds in the cell. It is also possible to use organic bases such as guanidine to precipitate polyphosphates selectively from their aqueous solutions. However, a PolyP assay according to the labile phosphorus of barium and other water-insoluble salts is possible but not fully reliable, because not all condensed phosphates are precipitated as barium salts.