By N.U. Bang
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The seriously acclaimed laboratory general, tools in Enzymology, is likely one of the such a lot hugely revered courses within the box of biochemistry. given that 1955, each one quantity has been eagerly awaited, often consulted, and praised via researchers and reviewers alike. The sequence comprises a lot fabric nonetheless proper at the present time - actually a vital ebook for researchers in all fields of existence sciences
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Additional resources for Thrombosis and Bleeding Disorders. Theory and Methods
A) Antiplasma thromboplastins: T h e instabil ity of the activity obtained in the thrombo plastin generation test has been studied by several investigators (Spaet and Gamer, 1955; Egli et al. 1957; Deutsch and Fuchs, 1958; Deutsch and Mammen, 1958). T w o fractions of plasma were found to inhibit plasma throm boplastin activity, one with an immediate effect, and one with a progressive type of action (Deutsch and Mammen, 1958). These two activities also differed in their precipitation characteristics and in some of their physical properties (Deutsch and Mammen, 1958).
These conflicting results may be due to differences in test systems (Mustard et al. 1962); differences in the colloidal state of the phospholipid par ticles; differences in p H or ionic strength (Hoelzl-Wallach et al. 1959); the state of pu rity or rate of decomposition of the various phospholipids (Daemen et al. 1965). T h e de gree of unsaturation and the localization of the fatty acid constituents on the phospholipids may also have contributed to these discrepan cies (Rouser and Schloredt, 1958).
Marcus and Zucker (1965) hypothesized that a protein which may either be an inherent part of the platelet membrane or an adsorbed plas ma protein, as suggested by Rodman et al. (1962), might be removed from the platelets during the early steps of coagulation. T h e re moval of this protein might enable the platelet membranes to interact with certain coagula tion factors. Another possibility is that Hage man factor (factor Χ Π ) which is apparently adsorbed on the platelet surface (Sharp, 1958; Waaler, 1959; Jürgens, 1962; latridis and Fer guson, 1965) becomes activated.