Download Vitamins and Coenzymes Part E by Nathan P. Kaplan, Nathan P. Colowick, Donald B. McCormick, PDF

By Nathan P. Kaplan, Nathan P. Colowick, Donald B. McCormick, Lemuel D. Wright

The seriously acclaimed laboratory general, Methods in Enzymology, is without doubt one of the such a lot hugely revered guides within the box of biochemistry. in view that 1955, every one quantity has been eagerly awaited, often consulted, and praised through researchers and reviewers alike. The sequence comprises a lot fabric nonetheless proper this present day - actually an important book for researchers in all fields of lifestyles sciences

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Vitamins and Coenzymes Part E

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3 × 103 l . m o l - l . c m - ' . 71 (4) Values are Yc_ SD; the numbers in parentheses give the number of different NADH preparations in which the parameters were determined. ) or a C~try 17, respectively. ). Residual Az40nm (%) A~0nmafter enzymic oxidation x 100 A 340nmbefore oxidation The measurements were carried out with a VSU II spectrophotometer against blanks that contained all components of the test except NADH. Corrections were done for changes of the volume and of the absorbance due to enzyme addition.

2116, 121 (1954)]. 36 NICOTINIC ACID: ANALOGS AND COENZYMES [5] T A B L E II PEAK CONDUCTIV1TIES OF ANIONIC COMPOUNDS ELUTED FROM DOWEX I-FORMATEa Compound 1. N M N +b 2. l-Methyl-4-pyridone-3carboxylic acid 3. 4-Hydroxynicotinic acid 4. Picolinic acid 5. Adenylyl (2' ~ 5') adenosine 6. Nicotinic acid 7. Nicotinuric acid 8. N A D + 9. a - N A D + 10. 1-Methyl-6-pyridone-3carboxylic acid 11. A M P 12. 2 ' - A M P 13. 6-Hydroxynicotinic acid 14. N A M N + (deamido N M N +) 15. 3'~AMW 16. 3',5'-Cyclic A M W 17.

The enzyme activity measured with the NADH preparation in question has to be compared with that obtained with pure NADH. To prepare pure NADH as reference, the TLC method described may be a suitable procedure. [5] I s o l a t i o n a n d A n a l y s i s o f P y r i d i n e N u c l e o t i d e s a n d Related Compounds by Liquid Chromatography By CARL BERNOFSKY Liquid chromatography is of proven value in the analysis of complex mixtures and is often utilized for the preparation of pure compounds.

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